{"id":1856,"date":"2026-09-11T08:15:52","date_gmt":"2026-09-11T08:15:52","guid":{"rendered":"https:\/\/nextrabiochem.eu\/produkt\/aicar-50mg\/"},"modified":"2026-09-11T12:16:04","modified_gmt":"2026-09-11T12:16:04","slug":"aicar-50mg","status":"publish","type":"product","link":"https:\/\/nextrabiochem.eu\/en\/produkt\/aicar-50mg\/","title":{"rendered":"Aicar 50mg"},"content":{"rendered":"<p><strong>AICAR<\/strong><\/p>\n<p><i>Synthetic small-molecule nucleoside analogue (5-aminoimidazole-4-carboxamide-1-<\/i>\u03b2<i>-D-ribofuranoside) for in vitro research on allosteric AMPK activation, GLUT4 glucose transporter translocation, PGC-1$alpha$ induction, and metabolic reprogramming in myocytes and hepatocytes<\/i><\/p>\n<p><strong>Overview &amp; Mechanism of Action (In Vitro)<\/strong><\/p>\n<p><strong>AICAR<\/strong> (chemically designated as <i>5-aminoimidazole-4-carboxamide-1-<\/i>\u03b2<i>-D-ribofuranoside<\/i>, also known as <i>Acadesine<\/i> or <i>Z-riboside<\/i>) is a synthetic, membrane-permeable nucleoside precursor (non-peptidic) functioning as a direct structural analogue of adenosine monophosphate (AMP). Upon cellular uptake via equilibrative nucleoside transporters (ENTs), it is intracellularly phosphorylated by adenosine kinase into its bioactive monophosphate metabolite (<strong>ZMP<\/strong>). ZMP binds directly to the \u03b3-subunit of AMP-activated protein kinase (<strong>AMPK<\/strong>), allosterically mimicking cellular energy depletion. In cellular bioenergetics, molecular diabetology, and metabolic physiology, AICAR serves as the reference standard for investigating cellular energy sensing networks:<\/p>\n<ul>\n<li><strong>Allosteric Activation &amp; Phosphorylation of AMPK:<\/strong> ZMP binding induces an allosteric conformational shift that activates AMPK kinase activity while protecting the critical regulatory residue Thr172 from dephosphorylation by protein phosphatases, facilitating maximal activation by upstream kinases (such as LKB1).<\/li>\n<li><strong>Stimulation of Insulin-Independent GLUT4 Translocation:<\/strong> AMPK activation directly induces the recruitment and fusion of GLUT4 glucose storage vesicles to the plasma membrane in skeletal myotubes, driving glucose uptake in vitro.<\/li>\n<li><strong>Inhibition of Lipogenesis &amp; Acceleration of <\/strong>\u03b2<strong>-Oxidation:<\/strong> Direct phosphorylation and inactivation of acetyl-CoA carboxylase (ACC) decreases intracellular malonyl-CoA levels, relieving the inhibition of carnitine palmitoyltransferase-1 (CPT-1) and promoting mitochondrial fatty acid uptake.<\/li>\n<li><strong>Induction of Mitochondrial Biogenesis via PGC-1$alpha$:<\/strong> Upregulates transcriptional coactivator PGC-1$alpha$ and downstream nuclear respiratory factors (NRF-1\/2), increasing mitochondrial density and oxidative capacity in cell cultures.<\/li>\n<\/ul>\n<p><strong>Technical &amp; Chemical Specifications<\/strong><\/p>\n<ul>\n<li><strong>Classification:<\/strong> Research Small Molecule \/ Direct AMPK Activator (AMP Analogue)<\/li>\n<li><strong>Chemical Name:<\/strong> 5-amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]imidazole-4-carboxamide<\/li>\n<li><strong>Molecular Formula:<\/strong> C9H14N4O5<\/li>\n<li><strong>Molecular Weight:<\/strong> \u2248258.23\u00a0g\/mol<\/li>\n<li><strong>Form:<\/strong> Sterile white to off-white crystalline \/ lyophilized powder<\/li>\n<li><strong>Component Identification:<\/strong> Verified via Mass Spectrometry (MS), NMR spectroscopy, and analytical HPLC<\/li>\n<li><strong>Recommended Dissolution Protocol (Hydrophilic Nucleoside):<\/strong> AICAR exhibits good aqueous solubility. The recommended primary solvents for in vitro stock preparation are sterile deionized water, sterile phosphate-buffered saline (PBS, pH 7.2\u20137.4), or DMSO. Prepare stock solutions and dilute directly into cell culture media.<\/li>\n<li><strong>Storage Conditions:<\/strong> Store long-term at -20 \u00b0C in a dry, dark environment in a tightly sealed container. Aqueous or DMSO stock solutions should be stored in aliquots at -80 \u00b0C (or -20 \u00b0C), protected from repetitive freeze-thaw cycles.<\/li>\n<\/ul>\n<p><strong>Scientific References &amp; Literature<\/strong><\/p>\n<ul>\n<li><i>Corton, J. M., et al.<\/i> 5-Aminoimidazole-4-carboxamide ribonucleoside: A specific method for activating AMP-activated protein kinase in intact cells? <i>European Journal of Biochemistry<\/i>, 1995.<\/li>\n<li><i>Hardie, D. G., et al.<\/i> AMP-activated protein kinase: A key system for the control of cellular and whole-body energy balance. <i>Physiological Reviews<\/i>, 2012.<\/li>\n<li><i>Merrill, G. F., et al.<\/i> AICA riboside increases AMP-activated protein kinase, fatty acid oxidation, and glucose uptake in rat muscle. <i>American Journal of Physiology-Endocrinology and Metabolism<\/i>, 1997.<\/li>\n<li><i>Narkar, V. A., et al.<\/i> AMPK and PPARdelta agonists are exercise mimetics providing functional endurance benefits in cellular and animal models. <i>Cell<\/i>, 2008.<\/li>\n<\/ul>\n<p><strong>Legal Disclaimer &amp; Terms of Purchase<\/strong><\/p>\n<p>This product is classified and supplied <strong>strictly for laboratory, scientific, and in vitro research purposes only (Research Use Only \u2013 RUO)<\/strong>. This compound is not a drug, medicine, dietary supplement, cosmetic product, or substance intended for human or veterinary consumption, direct administration, injection, or diagnostic procedures.<\/p>\n<p>By placing an order, the purchaser explicitly confirms that they are <strong>at least 18 years of age<\/strong>, possess the requisite professional qualifications and laboratory equipment to safely handle research materials, and are fully conversant with all relevant safety protocols for managing research substances. The seller assumes no liability for any use contrary to these terms.<\/p>\n","protected":false},"excerpt":{"rendered":"<ul>\n<li><strong>Intended Use:<\/strong> This product is supplied <strong>strictly for laboratory, scientific, and research purposes only (Research Use Only \/ in vitro)<\/strong>. It is not a medicinal product, food, or dietary supplement. The substance is not intended for human or animal consumption, administration, or therapeutic use.<\/li>\n<li><strong>Form &amp; Contents:<\/strong> Supplied as a sterile lyophilized powder in a sealed laboratory vial. Does not include reconstitution solvent unless explicitly stated.<\/li>\n<li><strong>Recommended Storage:<\/strong> To maintain maximum stability, store the unopened vial in a cool, dry, and dark place at 2\u20138 \u00b0C (recommended at -20 \u00b0C for long-term storage).<\/li>\n<li><strong>Illustrative Product Image:<\/strong> Product images are for <strong>illustrative purposes only<\/strong>. The actual appearance of the vial, flip-off cap color, or laboratory label design may vary depending on the production batch.<\/li>\n<\/ul>\n","protected":false},"featured_media":1857,"template":"","meta":[],"product_brand":[],"product_cat":[47],"product_tag":[],"class_list":["post-1856","product","type-product","status-publish","has-post-thumbnail","product_cat-lyophilized-compounds","first","instock","purchasable","product-type-simple"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Aicar 50mg - Nextrabiochem.eu<\/title>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Aicar 50mg - Nextrabiochem.eu\" \/>\n<meta property=\"og:description\" content=\"Intended Use: This product is supplied strictly for laboratory, scientific, and research purposes only (Research Use Only \/ in vitro). 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