Description

P21 (Peptide P21 / P021)

Synthetic bioactive peptide derivative of Ciliary Neurotrophic Factor (CNTF 147–150 epitope / Ac-DGGL-NH2) for in vitro research on dentate gyrus neurogenesis, tau hyperphosphorylation inhibition, BDNF/TrkB axis signaling, and synaptic plasticity

Overview & Mechanism of Action (In Vitro)

P21 (scientifically designated as P021 or Ac-Asp-Gly-Gly-Leu-NH2 derivative) is a synthetic neurogenic peptide engineered from the biologically active binding domain of Ciliary Neurotrophic Factor (CNTF, specifically the 147–150 amino acid epitope). It is designed to overcome the classical limitations of full-length CNTF by neutralizing neutralizing antibodies and avoiding unwanted gp130/LIFR pathway activation (which triggers peripheral inflammation in cellular assays). In molecular neurobiology, neurodegenerative research, and synaptic biology, it serves as a key reference compound for studying hippocampal restoration:

• Stimulation of Adult Neurogenesis in the Dentate Gyrus: Markedly enhances the proliferation, self-renewal, and neuronal differentiation of neural stem cells (NSCs) and neural progenitor cells into mature, functional neurons in hippocampal cultures.
• Upregulation of the BDNF / TrkB Signaling Cascade: Induces brain-derived neurotrophic factor (BDNF) expression and stimulates its high-affinity receptor TrkB, driving downstream pro-survival MAPK/ERK and PI3K/Akt intracellular cascades.
• Inhibition of GSK-3$beta$ & Reduction of Tau Hyperphosphorylation: Upregulates the inhibitory phosphorylation of glycogen synthase kinase-3 beta (GSK-3$beta$ at Ser9), mitigating aberrant tau hyperphosphorylation and preserving microtubule structural stability.
• Enhancement of Synaptic Density & Plasticity Markers: Elevates levels of essential pre- and post-synaptic markers, including synaptophysin and PSD-95, reflecting the reinforcement of dendritic arborization and synaptic connectivity in vitro.

Technical & Chemical Specifications

• Classification: Research Peptide / CNTF-Derived Neurogenic Peptide (P021 Analogue)
• Amino Acid Sequence / Structure: Ac-Asp-Gly-Gly-Leu-NH2 (Ac-DGGL-NH2 / lipophilic adamantane-stabilized derivative)
• Form: Sterile lyophilized white powder (cake)
• Component Identification: Verified via Mass Spectrometry (MS) and analytical HPLC
• Recommended Reconstitution Solvent: Sterile bacteriostatic water or sterile phosphate-buffered saline (PBS, pH 7.2–7.4). For lipophilic modifications, a 0.1% sterile aqueous acetic acid solution (acetic acid water) can be employed.
• Storage Conditions: Store long-term at -20 °C in a dry, dark environment. Following reconstitution, keep refrigerated at 2–8 °C in accordance with standard laboratory protocols.

Scientific References & Literature

• Kazim, S. F., et al. Disease-modifying effect of chronic oral treatment with a neurogenic and neurotrophic compound (P021) in Alzheimer’s disease models. Neurobiology of Disease, 2014.
• Baazaoui, N., & Iqbal, K. A small molecule neurogenic compound (P021) enhances synaptic plasticity and rescues cognitive impairment by inhibiting GSK-3beta. Journal of Alzheimer’s Disease, 2017.
• Li, B., et al. Inhibition of Tau hyperphosphorylation and enhancement of neurogenesis by a CNTF-derived peptide. Neurochemistry International, 2010.
• Khatoon, S., et al. A non-toxic CNTF-derived peptide stimulates neurogenesis and improves synaptic connectivity in vitro. FEBS Letters, 2007.

Legal Disclaimer & Terms of Purchase

This product is classified and supplied strictly for laboratory, scientific, and in vitro research purposes only (Research Use Only – RUO). This compound is not a drug, medicine, dietary supplement, cosmetic product, or substance intended for human or veterinary consumption, direct administration, injection, or diagnostic procedures.

By placing an order, the purchaser explicitly confirms that they are at least 18 years of age, possess the requisite professional qualifications and laboratory equipment to safely handle research materials, and are fully conversant with all relevant safety protocols for managing research substances. The seller assumes no liability for any use contrary to these terms.