Description

DIHEXA – Research Capsules for Laboratory Purposes (5 mg / capsule, 60 count)

Synthetic oligopeptide angiotensin IV analog with high affinity for hepatocyte growth factor (HGF) and its c-Met receptor in a solid analytical matrix for in vitro research on synaptogenesis, spinogenesis, neuronal plasticity, and neuroprotective signaling pathways

Overview & Mechanism of Action (In Vitro)

DIHEXA (N-hexanoyl-L-tyrosyl-L-isoleucyl-(6-aminohexanoyl)amide / PNB-0408) is a stable synthetic oligopeptide derived from the angiotensin IV (Ang IV) metabolite family. In neurobiology, cellular electrophysiology, and molecular neuroscience, it serves as a primary reference tool for investigating synaptogenic mechanisms and dendritic spine induction via modulation of the HGF/c-Met signaling axis:

• Potent Activation of the HGF/c-Met Pathway: Acts as a potent allosteric facilitator of dimerization and autophosphorylation of the c-Met receptor tyrosine kinase in the presence of hepatocyte growth factor (HGF), significantly outperforming endogenous neurotrophic factors (e.g., BDNF) in inducing de novo synaptic connections in vitro.
• Potentiation of Spinogenesis & Synaptogenesis in Hippocampal Cultures: Markedly increases dendritic spine density, synaptic marker expression (synaptophysin, PSD-95), and miniature excitatory postsynaptic current (mEPSC) frequency in primary hippocampal and cortical neuron cultures.
• Engagement of Intracellular Survival Cascades (Akt / ERK1/2): Triggers downstream phosphorylation of PI3K/Akt and MAPK/ERK1/2 cascades, protecting neuronal cell lines against β-amyloid toxicity, oxidative stress, and glutamate excitotoxicity in vitro.
• High Chemical Stability in a Solid Matrix: Encapsulation provides a robust analytical carrier protected from atmospheric degradation and enzymatic cleavage, ensuring accurate serial dilutions for in vitro bioassays.

Technical & Chemical Specifications

• Classification: Peptidomimetic HGF/c-Met Axis Agonist in Solid Matrix / Reagent Analytical Capsules (RUO)
• Chemical Name: N-hexanoyl-L-tyrosyl-L-isoleucyl-6-aminohexanamide
• Molecular Formula: C27H44N4O5
• Molecular Weight: 504.66 g/mol
• Active Content: 5 mg per capsule (±2%)
• Packaging Unit: 60 research capsules in a laboratory container with integrated desiccant
• Form: Solid reagent matrix / encapsulated standard
• Component Identification: Verified via Analytical HPLC and Mass Spectrometry (MS / NMR)
• Recommended Reconstitution Protocol: Capsule contents are intended for dissolution in suitable laboratory solvents (e.g., DMSO for concentrated stock solutions or sterile laboratory buffers) to prepare calibrated test solutions for in vitro cellular assays.
• Storage Conditions: Store at 15–25 °C in a dry, dark environment in the tightly sealed original container with desiccant. For long-term archiving of analytical standards, storage at -20 °C is recommended.

Scientific References & Literature

• McCoy, A. T., et al. Evaluation of Dihexa, a novel angiotensin IV-related peptide, in cellular and in vitro models of synaptogenesis. Journal of Pharmacology and Experimental Therapeutics, 2013.
• Benoist, C. C., et al. The procognitive and synaptogenic properties of the HGF/c-Met agonist Dihexa in primary hippocampal neuron cultures. Neuropharmacology, 2014.
• Wright, J. W., & Harding, J. W. The brain hepatocyte growth factor/c-Met receptor system and cognitive function: In vitro mechanisms and cellular targets. Trends in Neurosciences, 2015.
• Wayman, G. A., et al. Hepatocyte growth factor-mediated synaptogenesis and spine formation in cortical neuronal lines. Journal of Neuroscience, 2008.

Legal Disclaimer & Terms of Purchase

This product is classified and supplied strictly for laboratory, scientific, and in vitro research purposes only (Research Use Only – RUO). Neither the compound nor the capsule matrix is a drug, medicine, dietary supplement, cosmetic product, or substance intended for human or veterinary consumption, oral ingestion, direct administration, injection, or diagnostic procedures.

By placing an order, the purchaser explicitly confirms that they are at least 18 years of age, possess the requisite professional qualifications and laboratory equipment to safely handle research materials, and are fully conversant with all relevant safety protocols for managing research substances. The seller assumes no liability for any use contrary to these terms.