Description

NAD+ – Research Capsules for Laboratory Purposes (500 mg / capsule, 60 count)

Highly purified bioactive coenzyme nicotinamide adenine dinucleotide (β-NAD+) in a solid analytical matrix for in vitro research on cellular bioenergetics, sirtuin activity (SIRT1–SIRT7), PARP enzyme function, mitochondrial oxidative metabolism, and DNA repair mechanisms

Overview & Mechanism of Action (In Vitro)

NAD+ (β-nicotinamide adenine dinucleotide) is an essential intracellular coenzyme and central redox cofactor present across all living cells. In molecular biology, cellular bioenergetics, and aging research, it serves as a critical substrate for NAD+-consuming enzymes that regulate epigenetic modifications, genomic stability, and mitochondrial respiration. Encapsulation into a standardized solid matrix ensures high cofactor stability, prevents spontaneous oxidation and moisture absorption, and allows accurate aliquot weighing for laboratory testing:

• Obligate Co-Substrate for Sirtuin Enzymes (SIRT1–SIRT7): Serves as an essential cofactor for NAD+-dependent protein deacetylases (SIRT1, SIRT3), regulating downstream transcription factors (e.g., PGC-1$alpha$, FOXO) that drive mitochondrial biogenesis, oxidative phosphorylation, and cellular antioxidant responses in vitro.
• Activation of PARP Enzymes in DNA Damage Repair: Acts as an ADP-ribose donor for poly(ADP-ribose) polymerases (particularly PARP-1), which are required for single-strand break detection and the orchestration of enzymatic repair cascades under genotoxic stress.
• Electron Transfer in the Mitochondrial Respiratory Chain: Maintains the central NAD+/NADH redox couple essential for driving glycolysis, the tricarboxylic acid (TCA) cycle, and complex I electron transport during ATP generation.
• Modulation of CD38/CD157 & Calcium Signaling: Participates in the enzymatic synthesis of cyclic ADP-ribose (cADPR), a key secondary messenger modulating intracellular calcium (Ca2+) mobilization.

Technical & Chemical Specifications

• Classification: Intracellular Coenzyme / Bioenergetics Analytical Standard in Solid Matrix (RUO)
• Chemical Name: β-Nicotinamide adenine dinucleotide (oxidized form)
• Molecular Formula: C21H27N7O14P2
• Molecular Weight: 663.43 g/mol
• Active Content: 500 mg per capsule (±2%)
• Packaging Unit: 60 research capsules in a laboratory container with integrated desiccant
• Form: Solid reagent matrix / encapsulated standard
• Component Identification: Verified via Analytical HPLC and Spectrophotometric Assay (absorbance at 260 nm)
• Recommended Reconstitution Protocol: Capsule contents are intended for dissolution in sterile laboratory buffers (e.g., sterile PBS pH 7.2–7.4) or sterile deionized water to prepare calibrated working solutions for in vitro cellular assays. Reconstituted aqueous solutions should be used immediately or stored frozen to prevent spontaneous hydrolysis.
• Storage Conditions: Store at 15–25 °C in a dry, dark environment in the tightly sealed original container with desiccant. For long-term archiving of analytical standards, storage at -20 °C is recommended.

Scientific References & Literature

• Imai, S., & Guarente, L. NAD+ and sirtuins in aging and disease. Trends in Cell Biology, 2014.
• Cantó, C., et al. NAD+ metabolism and the control of energy homeostasis: A balancing act between mitochondria and the nucleus. Cell Metabolism, 2015.
• Verdin, E. NAD⁺ in aging, metabolism, and neurodegeneration. Science, 2015.
• Bai, P., et al. Poly(ADP-ribose) polymerases as modulators of cellular energy metabolism and DNA damage response. Molecular Cell, 2011.

Legal Disclaimer & Terms of Purchase

This product is classified and supplied strictly for laboratory, scientific, and in vitro research purposes only (Research Use Only – RUO). Neither the compound nor the capsule matrix is a drug, medicine, dietary supplement, cosmetic product, or substance intended for human or veterinary consumption, oral ingestion, direct administration, injection, or diagnostic procedures.

By placing an order, the purchaser explicitly confirms that they are at least 18 years of age, possess the requisite professional qualifications and laboratory equipment to safely handle research materials, and are fully conversant with all relevant safety protocols for managing research substances. The seller assumes no liability for any use contrary to these terms.