Description

PNC-27 – Lyophilized Peptide for In Vitro Research (5 mg) Synthetic chimeric peptide composed of a p53 tumor-suppressor protein fragment and a transfection vector (penetratin domain motif) in the form of high-purity lyophilisate for in vitro research on membrane protein interactions, oncological model cell lines, and selective cytotoxic mechanisms.

Overview and Mechanism of Action (In Vitro) PNC-27 is a specialized peptide sequence that serves in oncological research and cell biology as a reference tool for studying targeted membrane interaction and inducing necrosis in transformed cells:

• Interaction with HDM-2 in the cell membrane in vitro: Binds to human double minute 2 (HDM-2) protein, which is expressed on the surface of the plasma membrane in a range of cancer cells.
• Induction of membrane perforation and cell necrosis in vitro: Due to its binding to membrane HDM-2, it specifically disrupts the integrity of the plasma membrane of cancer cells, leading to their selective lysis and necrosis, while healthy cells without this expression remain primarily unaffected.
• Study of anti-tumor signaling pathways: Used to analyze differences in sensitivity between immortalized/malignant lines and primary untransformed cultures in a controlled laboratory environment.
• Lyophilized format for maximum stability: The product is supplied as a stable lyophilized powder, ensuring long-term purity and stability prior to reconstitution and allowing precise preparation of working solutions for laboratory protocols.

Technical and Chemical Specification

• Classification: Chimeric p53-penetratin peptide / Anti-tumor research standard / Research peptide (RUO)
• Product format: Lyophilized powder in a glass vial
• Substance content: 5 mg of pure lyophilisate per vial
• Amino acid sequence: Pro-Pro-Glu-Leu-Ser-Gln-Glu-Phe-Phe-Cys-Glu-Trp-Leu-Gln-Val-Glu-Ser-Thr-Leu-Arg-Cys-Glu (linked to penetratin sequence RQIKIWFQNRRMKWKK)
• Form: Lyophilized powder / highly purified biomolecule intended for reconstitution under laboratory conditions
• Recommended reconstitution: For in vitro use, reconstitute in a sterile buffered solution (e.g., PBS) or sterile deionized water for laboratories according to the specific protocol.
• Storage conditions: Store lyophilized powder at –20 °C (short-term at 2–8 °C in a dark and dry place). Protect from moisture and freezing. After reconstitution, keep refrigerated and consume according to laboratory standards.

Professional Sources and Literature

• Sareen, D., et al. PNC-27, a p53-based peptide, induces necrosis of human cancer cells by binding to HDM2 located in their plasma membranes. Cancer Research, 2007.
• Michl, J., et al. p53-derived peptide PNC-27 induces apoptosis and necrosis of cancer cells. International Journal of Cancer, 2006.

Legal Disclaimer and Terms of Purchase This product is classified and supplied exclusively for laboratory, scientific, and in vitro research purposes (Research Use Only – RUO). The lyophilized peptide is not a medicinal product, drug, weight-loss preparation, dietary supplement, or substance intended for human or veterinary consumption, oral ingestion, injection, or diagnostic procedures. By submitting an order, the buyer explicitly confirms that they have reached the age of at least 18 years, possess adequate laboratory infrastructure and personnel equipment for safe handling of research chemicals, and are fully familiar with laboratory safety protocols. The seller assumes no liability for any improper or unapproved use.