Description

Bacteriostatic Phosphate-Buffered Saline for Laboratory Research (PBS with 0.9% Benzyl Alcohol)

Sterile isotonic laboratory reconstitution and buffering medium (pH 7.4) containing 0.9% benzyl alcohol for in vitro dissolution and stabilization of pH-sensitive lyophilized peptides, proteins, and antibodies with microbial proliferation inhibition during multi-dose sampling

Overview & Functional Role in Laboratory Research (In Vitro)

Bacteriostatic Phosphate-Buffered Saline (Bacteriostatic PBS / PBS with 0.9% benzyl alcohol) is a highly purified, sterile, and non-pyrogenic laboratory medium combining a precisely balanced isotonic phosphate buffer system with the antimicrobial protection of 0.9% (9 mg/mL) benzyl alcohol (). Unlike unpreserved standard PBS or non-ionic bacteriostatic water, this medium provides physiological pH control and constant ionic strength, which is essential for compounds susceptible to degradation or aggregation in unbuffered systems:

• Strict Physiological pH Stabilization (7.4): The phosphate buffering system reliably maintains a stable pH range of 7.2–7.4, preventing precipitation, deamidation, or acid-induced degradation of peptides sensitive to subtle pH shifts.
• Inhibition of Microbial Proliferation During Multi-Access Sampling: The inclusion of 0.9% benzyl alcohol suppresses the growth of a broad spectrum of bacteria and fungi, enabling safe long-term storage and repeated aliquot withdrawals from a single stock vial at 2–8 °C.
• Preservation of Native Protein & Peptide Conformation: The isotonic salt balance (, , , ) mimics physiological osmotic conditions, safeguarding secondary and tertiary macromolecular structures.
• Broad Biochemical Compatibility: An ideal solvent medium for long-term in vitro kinetic experiments, binding assays, receptor-ligand interactions, and immunochemical procedures requiring a sterile, buffered environment.

Technical & Chemical Specifications

• Classification: Specialized Laboratory Buffering Medium / Bacteriostatic Reconstitution Reagent for Research (RUO)
• Composition:  (137 mM , 2.7 mM , 10 mM , 1.8 mM ), ultra-pure sterile deionized water, 0.9% benzyl alcohol (9 mg/mL)
• pH Value: 7.4  0.1 (at 25 °C)
• Osmolarity:  (isotonic profile)
• Form: Clear, colorless sterile liquid solution
• Quality Standard: 0.22  membrane filtered, endotoxin-free / non-pyrogenic grade
• Storage Conditions: Store unopened at 15–25 °C (room temperature) in a dry, dark environment. Following initial container access, store refrigerated at 2–8 °C in compliance with standard Good Laboratory Practice (GLP) protocols. Protect from freezing.

Scientific References & Literature

• Sambrook, J., & Russell, D. W. Molecular Cloning: A Laboratory Manual – Phosphate-Buffered Saline Preparation and Preservation Standards. Cold Spring Harbor Laboratory Press, 2001.
• Powell, M. F. Peptide stability in aqueous parenteral formulations: Impact of buffer capacity and antimicrobial preservatives. Journal of Pharmaceutical Science & Technology, 1993.
• Manning, M. C., et al. Stability of protein and peptide reagents: Physical and chemical degradation pathways in buffered media. Pharmaceutical Research, 2010.
• European Pharmacopoeia (Ph. Eur.). Reagents and Buffer Solutions: Isotonic Phosphate Buffers for Laboratory Analyses.

Legal Disclaimer & Terms of Purchase

This product is classified and supplied strictly for laboratory, scientific, and in vitro research purposes only (Research Use Only – RUO). This compound is not a drug, medicine, dietary supplement, cosmetic product, or substance intended for human or veterinary consumption, direct administration, injection, or diagnostic procedures.

By placing an order, the purchaser explicitly confirms that they are at least 18 years of age, possess the requisite professional qualifications and laboratory equipment to safely handle research materials, and are fully conversant with all relevant safety protocols for managing research substances. The seller assumes no liability for any use contrary to these terms.