Description

ACE-031 – Lyophilized Peptide for In Vitro Research (1 mg) Synthetic recombinant fusion protein (activin receptor type IIB / ActRIIB-Fc) in the form of high-purity lyophilisate for in vitro research on myostatin signaling pathways, muscle mass regulation, osteogenesis, growth factor blockade, and cell proliferation in muscle and bone cell cultures.

Overview and Mechanism of Action (In Vitro) ACE-031 is designed as a soluble decoy receptor (Fc fusion protein) that, in cell biology, muscle physiology, and tissue engineering research, serves as a reference inhibitor of negative regulators of muscle growth:

• Neutralization of myostatin and TGF-beta family ligands in vitro: Specifically binds to myostatin (GDF-8), GDF-11, and other related proteins, effectively blocking their interaction with natural cellular receptors (ActRIIB) on the surface of myocytes.
• Study of hypertrophic signaling pathways: In in vitro muscle cell cultures, it allows for the investigation of the activation of anabolic pathways (e.g., Akt/mTOR signaling cascades) and the suppression of atrophic gene programs without directly affecting insulin resistance.
• Bone metabolism and osteogenesis research: Used to analyze cross-talk between muscle mass regulation and bone tissue mineralization in model co-cultures of osteoblasts and osteoclasts.
• Lyophilized format for maximum stability: The product is supplied as a stable lyophilized powder, ensuring long-term purified stability prior to reconstitution and allowing precise dilution of working solutions for laboratory tests.

Technical and Chemical Specification

• Classification: Fusion protein / Myostatin inhibitor / Research peptide standard (RUO)
• Product format: Lyophilized powder in a glass vial
• Substance content: 1 mg of pure lyophilisate per vial
• Structure: Recombinant fusion protein composed of the extracellular domain of the human ActRIIB receptor linked to the Fc domain of human IgG1
• Form: Lyophilized powder / highly purified biomolecule intended for reconstitution under laboratory conditions
• Recommended reconstitution: For in vitro use, reconstitute in a sterile buffered solution (e.g., PBS containing a carrier protein according to a specific laboratory protocol).
• Storage conditions: Store lyophilized powder at –20 °C (short-term at 2–8 °C in a dark and dry place). Protect from moisture and freezing. After reconstitution, handle gently and consume according to laboratory standards (avoid repeated freezing).

Professional Sources and Literature

• Attie, K. M., et al. A soluble activin type IIA receptor (ACE-031) increases muscle mass and bone mineral density in preclinical models. Muscle & Nerve, 2010.
• Campbell, J. A., et al. Inhibition of myostatin and activin signaling by soluble ActRIIB-Fc. Molecular Endocrinology, 2017.
• Latres, E., et al. Activin A more than myostatin underlies the mechanisms of myostatin pathway inhibition via ActRIIB-Fc. Skeletal Muscle, 2017.

Legal Disclaimer and Terms of Purchase This product is classified and supplied exclusively for laboratory, scientific, and in vitro research purposes (Research Use Only – RUO). The lyophilized fusion protein is not a medicinal product, drug, weight-loss preparation, dietary supplement, or substance intended for human or veterinary consumption, oral ingestion, injection, or diagnostic procedures. By submitting an order, the buyer explicitly confirms that they have reached the age of at least 18 years, possess adequate laboratory infrastructure and personnel equipment for safe handling of research chemicals, and are fully familiar with laboratory safety protocols. The seller assumes no liability for any improper or unapproved use.