Description

ARA-290

Synthetic 11-amino acid erythropoietin-derived peptide (cibinetide / pyroglutamate analogue) for in vitro research on innate repair receptor (IRR / EPOR-βcR) activation, neuroprotection, neuropathic modulation, and tissue repair without erythropoiesis

Overview & Mechanism of Action (In Vitro)

ARA-290 (also designated as Cibinetide) is a synthetic linear 11-amino acid peptide with the sequence (pGlu-Glu-Gln-Leu-Glu-Arg-Ala-Leu-Asn-Ser-Ser), engineered based on the spatial helix B domain of native human erythropoietin (EPO). It selectively activates tissue-protective pathways without engaging the classic homodimeric erythropoietin receptor (EPOR2) responsible for hematopoiesis and thrombogenesis. In neurobiology, immunology, and peripheral neuropathy research, it serves as a primary reference model for investigating axonal repair and cytoprotective mechanisms:

• Selective Activation of the Innate Repair Receptor (IRR): High-affinity binding to the heterodimeric receptor complex formed by the erythropoietin receptor and the common β-subunit (EPOR-βcR / CD131), locally upregulated upon cellular stress, ischemia, or metabolic injury.
• Neuroprotection & Small Nerve Fiber Repair: Stimulation of neurite outgrowth, cytoprotection of neurons and Schwann cells against apoptotic triggers, and evaluation of intraepidermal nerve fiber density (IENFD) restoration in vitro.
• Anti-Inflammatory Modulation & NF-κB Suppression: Downregulation of the transcription factor NF-κB in macrophages and glial cells, resulting in attenuated pro-inflammatory cytokine release (TNF-α, IL-1$beta$, IL-6) and reduced inducible nitric oxide synthase (iNOS) expression.
• Non-Erythropoietic Profile: Total lack of cross-reactivity with classical erythroid progenitor cells, eliminating the risk of polycythemia or procoagulant alterations in experimental models.

Technical & Chemical Specifications

• Classification: Research Peptide / Innate Repair Receptor (IRR) Agonist (Cibinetide)
• Amino Acid Sequence: Pyr-Glu-Gln-Leu-Glu-Arg-Ala-Leu-Asn-Ser-Ser (Pyr = pyroglutamate / pGlu)
• Form: Sterile lyophilized white powder (cake)
• Component Identification: Verified via Mass Spectrometry (MS) and analytical HPLC
• Recommended Reconstitution Solvent: Sterile bacteriostatic water or sterile phosphate-buffered saline (PBS)
• Storage Conditions: Store long-term at -20 °C in a dry, dark environment. Following reconstitution, keep refrigerated at 2–8 °C in accordance with standard laboratory protocols.

Scientific References & Literature

• Brines, M., et al. ARA 290, a peptide derived from erythropoietin, activates the innate repair receptor and alleviates neuropathic pain in tissue models. Proceedings of the National Academy of Sciences, 2008.
• Dahan, A., et al. ARA 290 stimulates nerve fiber density and mitigates inflammation without stimulating erythropoiesis. Molecular Medicine, 2013.
• van Velzen, M., et al. The tissue-protective properties of ARA 290: An in vitro and clinical exploration in small fiber neuropathy. Expert Opinion on Investigational Drugs, 2014.
• Swartjes, M., et al. ARA 290, a nonerythropoietic peptide, attenuates inflammatory responses and microglial activation in models of tissue trauma. Journal of Neuroinflammation, 2014.

Legal Disclaimer & Terms of Purchase

This product is classified and supplied strictly for laboratory, scientific, and in vitro research purposes only (Research Use Only – RUO). This compound is not a drug, medicine, dietary supplement, cosmetic product, or substance intended for human or veterinary consumption, direct administration, injection, or diagnostic procedures.

By placing an order, the purchaser explicitly confirms that they are at least 18 years of age, possess the requisite professional qualifications and laboratory equipment to safely handle research materials, and are fully conversant with all relevant safety protocols for managing research substances. The seller assumes no liability for any use contrary to these terms.