Description

MGF (Mechano-Growth Factor / IGF-1Ec)

Synthetic bioactive C-terminal peptide isoform of Insulin-like Growth Factor-1 (IGF-1Ec) for in vitro research on mechanotransduction, satellite cell activation, myofiber regeneration, and tissue repair cascades

Overview & Mechanism of Action (In Vitro)

MGF (Mechano-Growth Factor, classified in humans as IGF-1Ec) is an alternatively spliced transcript variant of the Insulin-like Growth Factor-1 gene, naturally expressed in skeletal muscle and related tissues in response to mechanical loading, localized tension, or physical microtrauma. Unlike systemic hepatic IGF-1, MGF possesses a unique C-terminal E-domain extension that enables specialized local paracrine and autocrine regulatory actions. In molecular myology, tissue engineering, and cellular physiology, it serves as a primary reference standard for studying mechanobiology and localized tissue regeneration:

• Activation & Proliferation of Skeletal Muscle Satellite Cells: Stimulates quiescent myogenic stem cells (satellite cells) in injured or mechanically stressed muscle cultures in vitro, driving the initial expansion phase required for myofibril repair.
• Support of Myoblast Fusion & Myotube Hypertrophy: Induces the expression of transcription factors required for the fusion of committed myoblasts with existing myofibers while stimulating intracellular protein synthesis.
• Distinct E-Domain Signaling Pathway: Exerts bioactivity via specialized cellular signaling cascades independent of classic systemic IGF-1 receptor (IGF-1R) pathways, promoting pro-survival kinase activation and adaptive remodeling.
• Endothelial Proliferation & Angiogenesis Support: Promotes endothelial cell migration and network formation, facilitating microvascular restoration in damaged tissue culture models.

Technical & Chemical Specifications

• Classification: Research Peptide / C-Terminal IGF-1 Splice Variant (Mechano-Growth Factor)
• Form: Sterile lyophilized white powder (cake)
• Component Identification: Verified via Mass Spectrometry (MS) and analytical HPLC
• Recommended Reconstitution Solvent: Sterile bacteriostatic water or sterile phosphate-buffered saline (PBS, pH 7.2–7.4)
• Storage Conditions: Store long-term at -20 °C in a dry, dark environment. Following reconstitution, keep refrigerated at 2–8 °C in accordance with standard laboratory protocols.

Scientific References & Literature

• Goldspink, G. Research on mechanogrowth factor and muscle regeneration. Journal of Physiology, 2005.
• Yang, S., & Goldspink, G. Different splice variants of the IGF-I gene expressed in skeletal muscle and their respective roles in muscle growth. FEBS Letters, 2002.
• Hill, M., & Goldspink, G. Expression and differential control of MGF and IGF-I in contracting skeletal muscle. American Journal of Physiology-Cell Physiology, 2003.
• Matheny, R. W., et al. Properties of the IGF-1Ec (MGF) splice variant in skeletal muscle growth and repair. Journal of Applied Physiology, 2010.

Legal Disclaimer & Terms of Purchase

This product is classified and supplied strictly for laboratory, scientific, and in vitro research purposes only (Research Use Only – RUO). This compound is not a drug, medicine, dietary supplement, cosmetic product, or substance intended for human or veterinary consumption, direct administration, injection, or diagnostic procedures.

By placing an order, the purchaser explicitly confirms that they are at least 18 years of age, possess the requisite professional qualifications and laboratory equipment to safely handle research materials, and are fully conversant with all relevant safety protocols for managing research substances. The seller assumes no liability for any use contrary to these terms.