Description

NMN – Research Capsules for Laboratory Purposes (500 mg / capsule, 60 count)

Highly purified biologically active nucleotide (β-nicotinamide mononucleotide) in a solid analytical matrix for in vitro research on NAD+ salvage biosynthesis, sirtuin activation (SIRT1–SIRT7), PARP enzyme modulation, mitochondrial bioenergetics, and cellular senescence

Overview & Mechanism of Action (In Vitro)

NMN (β-nicotinamide mononucleotide / β-NMN) is a key bioactive nucleotide generated through the condensation of a nicotinamide moiety with ribose-5-phosphate catalyzed by nicotinamide phosphoribosyltransferase (NAMPT). In cellular bioenergetics, molecular biology, and gerontological research, it serves as a primary reference precursor and analytical standard for studying the regulation of the intracellular NAD+ metabolic pool and downstream enzymatic cascades across cultured cell models:

• Direct Intermediate in NAD+ Biosynthesis: Serves as the immediate substrate for nicotinamide mononucleotide adenylyltransferases (NMNAT1–3), directly elevating oxidized nicotinamide adenine dinucleotide (NAD+) levels and restoring the cellular NAD+/NADH redox ratio in vitro.
• Engagement of the Sirtuin Signaling Axis (SIRT1 / SIRT3): Enhanced availability of the NAD+ cofactor stimulates the deacetylase kinetics of nuclear and mitochondrial sirtuins, driving PGC-1$alpha$ deacetylation, transcriptional activation of metabolic programs, and mitochondrial biogenesis.
• Modulation of PARP Activity & DNA Repair Dynamics: Fuels poly(ADP-ribose) polymerases (primarily PARP-1) during cellular DNA damage detection and single-/double-strand break repair pathways in lines subjected to genotoxic stress.
• Optimization of Mitochondrial Respiration & Metabolic Plasticity: Restores mitochondrial membrane potential (ΔΨm), enhances oxygen consumption rates (OCR), and limits excessive reactive oxygen species (ROS) production in cellular senescence models in vitro.

Technical & Chemical Specifications

• Classification: NAD+ Biosynthetic Precursor / Nucleotide Standard in Solid Matrix (RUO)
• Chemical Name: β-nicotinamide mononucleotide (nicotinamide-D-ribonucleotide)
• Molecular Formula: C11H15N2O8P
• Molecular Weight: 334.22 g/mol
• Active Content: 500 mg per capsule (±2%)
• Packaging Unit: 60 research capsules in a laboratory container with integrated desiccant
• Form: Solid reagent matrix / encapsulated standard (β-anomer form)
• Component Identification: Verified via Analytical HPLC (purity >99%) and Mass Spectrometry (MS / NMR)
• Recommended Reconstitution Protocol: Capsule contents are intended for dissolution in sterile laboratory buffers (e.g., sterile PBS pH 7.2–7.4) or sterile deionized water to prepare calibrated working concentrations for in vitro cell culture assays.
• Storage Conditions: Store at 15–25 °C in a dry, dark environment in the tightly sealed original container with desiccant. For long-term archiving of analytical standards, storage at -20 °C is recommended.

Scientific References & Literature

• Yoshino, J., et al. Nicotinamide mononucleotide (NMN) increases NAD+ levels and rescues mitochondrial dysfunction in cellular models of metabolic stress. Cell Metabolism, 2011.
• Gomes, A. P., et al. Declining NAD+ induces a pseudohypoxic state disrupting nuclear-mitochondrial communication during aging in vitro. Cell, 2013.
• Imai, S., & Guarente, L. NAD+ and sirtuins in aging and disease: Mechanistic insights from in vitro cellular assays. Trends in Cell Biology, 2014.
• Tarantini, S., et al. Nicotinamide mononucleotide supplementation restores cerebromicrovascular endothelial function and mitigates oxidative stress in vitro. GeroScience, 2019.

Legal Disclaimer & Terms of Purchase

This product is classified and supplied strictly for laboratory, scientific, and in vitro research purposes only (Research Use Only – RUO). Neither the compound nor the capsule matrix is a drug, medicine, dietary supplement, cosmetic product, or substance intended for human or veterinary consumption, oral ingestion, direct administration, injection, or diagnostic procedures.

By placing an order, the purchaser explicitly confirms that they are at least 18 years of age, possess the requisite professional qualifications and laboratory equipment to safely handle research materials, and are fully conversant with all relevant safety protocols for managing research substances. The seller assumes no liability for any use contrary to these terms.