Description

KPV

Synthetic C-terminal tripeptide derived from α-MSH (Lys-Pro-Val) for in vitro research on anti-inflammatory signaling, NF-κB pathway inhibition, antimicrobial actions, and mucosal barrier integrity

Overview & Mechanism of Action (In Vitro)

KPV is a synthetic bioactive tripeptide consisting of three amino acids with the sequence (Lys-Pro-Val), representing the active C-terminal domain of the endogenous melanocyte-stimulating hormone (α-MSH). It retains the potent anti-inflammatory and cytoprotective properties of the parent peptide without inducing MC1R-mediated melanogenesis (pigmentation-free). In molecular immunology, gastroenterology, and dermatological research, it serves as a primary model for investigating anti-inflammatory signaling pathways:

• Inhibition of NF-κB Nuclear Translocation: Directly suppresses the phosphorylation and subsequent degradation of the inhibitory protein I$kappaBalpha$, preventing nuclear translocation of the transcription factor NF-κB and silencing downstream inflammatory gene transcription.
• Suppression of Pro-Inflammatory Cytokines & Mediators: Quantifiable reduction in the mRNA expression and release of major inflammatory markers (TNF-α, IL-1$beta$, IL-6, IL-8) as well as pro-inflammatory enzymes (iNOS, COX-2).
• PepT1-Mediated Uptake & Epithelial Barrier Integrity: Rapid cellular internalization via the oligopeptide transporter PepT1 (SLC15A1) and enhancement of tight junction assembly (zonula occludens-1, occludin) in intestinal and dermal epithelial models in vitro.
• Direct Antimicrobial & Antifungal Activity: Evaluation of direct pathogen membrane disruption against strains such as Staphylococcus aureus and Candida albicans without triggering classical drug-resistance mechanisms.

Technical & Chemical Specifications

• Classification: Research Tripeptide / C-Terminal α-MSH Fragment (11–13)
• Amino Acid Sequence: Lys-Pro-Val (KPV)
• Form: Sterile lyophilized white powder (cake)
• Component Identification: Verified via Mass Spectrometry (MS) and analytical HPLC
• Recommended Reconstitution Solvent: Sterile bacteriostatic water or sterile phosphate-buffered saline (PBS)
• Storage Conditions: Store long-term at -20 °C in a dry, dark environment. Following reconstitution, keep refrigerated at 2–8 °C in accordance with standard laboratory protocols.

Scientific References & Literature

• Catania, A., et al. The melanocortin system in leukocyte biology: Anti-inflammatory actions of the C-terminal tripeptide KPV. Journal of Leukocyte Biology, 2000.
• Dalmasso, G., et al. PepT1-mediated tripeptide KPV uptake reduces intestinal inflammation in cell culture and experimental colitis models. Gastroenterology, 2008.
• Luger, T. A., & Brzoska, T. α-MSH related tripeptides: Molecular mechanisms of anti-inflammatory actions in skin biology. Annals of the New York Academy of Sciences, 2007.
• Cutuli, M., et al. Antimicrobial effects of alpha-MSH and its C-terminal tripeptide KPV against Candida albicans and Staphylococcus aureus. Journal of Leukocyte Biology, 2000.

Legal Disclaimer & Terms of Purchase

This product is classified and supplied strictly for laboratory, scientific, and in vitro research purposes only (Research Use Only – RUO). This compound is not a drug, medicine, dietary supplement, cosmetic product, or substance intended for human or veterinary consumption, direct administration, injection, or diagnostic procedures.

By placing an order, the purchaser explicitly confirms that they are at least 18 years of age, possess the requisite professional qualifications and laboratory equipment to safely handle research materials, and are fully conversant with all relevant safety protocols for managing research substances. The seller assumes no liability for any use contrary to these terms.