Description

NAD+ – Pre-filled Laboratory Cartridge for In Vitro Research (500 mg / 3 ml)

Highly purified oxidized nicotinamide adenine dinucleotide () in a pre-formulated stabilized buffered solution for in vitro research on redox homeostasis, mitochondrial oxidative phosphorylation, sirtuin activation (SIRT1–SIRT7), and PARP enzymatic kinetics

Overview & Mechanism of Action (In Vitro)

NAD+ (-nicotinamide adenine dinucleotide, oxidized form) is a central cellular coenzyme and metabolic signaling molecule essential for hundreds of fundamental biochemical pathways. In molecular bioenergetics, mitochondrial physiology, and cellular senescence research, it serves as a primary analytical and experimental standard for investigating redox reactions and -dependent enzymatic cascades in cultured cell systems:

• Central Electron Carrier in Energy Metabolism: Acts as a primary electron acceptor in glycolysis, the tricarboxylic acid (TCA) cycle, and mitochondrial fatty acid -oxidation, maintaining the intracellular  redox potential to support oxidative phosphorylation in vitro.
• Obligate Cosubstrate for Sirtuins (SIRT1–SIRT7): Directly regulates the deacetylation kinetics of nuclear and mitochondrial sirtuins, driving downstream epigenetic modifications, PGC-1$\alpha$ activation, and mitochondrial biogenesis across cell models.
• Fueling PARP-Mediated DNA Damage Response: Provides the essential substrate pool for poly(ADP-ribose) polymerases (primarily PARP-1 and PARP-2) during chromatin remodeling and DNA strand break repair in lines subjected to genotoxic stress.
• Pre-formulated Format for Precise Laboratory Dispensing: The stabilized liquid matrix within a 3 ml cartridge eliminates manual dissolution variability, ensuring exceptional homogeneity and concentration consistency for automated and semi-automated micro-dispensing systems.

Technical & Chemical Specifications

• Classification: Pyridine Nucleotide Coenzyme / Redox Analytical Standard / Research Solution (RUO)
• Product Format: Pre-filled calibrated laboratory cartridge (3 ml volume)
• Compound Strength: 500 mg / 3 ml (approx. 166.67 mg/ml concentration)
• Chemical Name: -nicotinamide adenine dinucleotide (oxidized form)
• Molecular Formula:
• Molecular Weight:
• Form: Sterile stabilized solution / Ready-to-Use (RTU)
• Solution Matrix: Sterile buffered isotonic solution for laboratory use (pH-optimized, requires no reconstitution or dilution)
• Compatibility: Compatible with standard laboratory dispensing pens and micro-dispensers for 3 ml cartridges (dispensing pen/injector is not included in the package)
• Storage Conditions: Store at 2–8 °C in a dark, refrigerated environment. Do not freeze. Maintain standard laboratory cold-chain protocols to preserve compound stability.

Scientific References & Literature

• Canto, C., et al. NAD+ metabolism and the control of energy homeostasis: A balancing act between mitochondria and the nucleus in cellular models. Cell Metabolism, 2015.
• Verdin, E. NAD+ in aging, metabolism, and neurodegeneration: Molecular mechanisms in cell culture systems. Science, 2015.
• Bai, P., et al. PARP-1 inhibition increases mitochondrial metabolism through SIRT1 activation in cultured cell lines. Cell Metabolism, 2011.
• Covarrubias, A. J., et al. Senescent cells promote tissue NAD+ decline during aging via activating CD38 in vitro. Nature Metabolism, 2020.

Legal Disclaimer & Terms of Purchase

This product is classified and supplied strictly for laboratory, scientific, and in vitro research purposes only (Research Use Only – RUO). The stabilized solution and mechanical cartridge are not drugs, medicines, weight-loss agents, dietary supplements, or cosmetic products, and are not intended for human or veterinary consumption, oral ingestion, direct administration, injection, or diagnostic procedures.

By placing an order, the purchaser explicitly confirms that they are at least 18 years of age, possess the requisite professional qualifications and laboratory infrastructure to safely handle research substances, and are fully conversant with all relevant laboratory safety protocols. The seller assumes no liability for any use contrary to these terms.