Description

5-Amino-1MQ – Research Capsules for Laboratory Purposes (50 mg / capsule, 60 count)

Synthetic selective membrane-permeable inhibitor of nicotinamide N-methyltransferase (NNMT) in a solid analytical matrix for in vitro research on cellular energy metabolism, intracellular NAD+ modulation, mitochondrial biogenesis, and adipocyte differentiation

Overview & Mechanism of Action (In Vitro)

5-Amino-1MQ (5-amino-1-methylquinolinium) is a synthetic, highly selective, membrane-permeable small molecule developed as a targeted inhibitor of the enzyme nicotinamide N-methyltransferase (NNMT). In cellular bioenergetics, molecular biology, and metabolic research, it serves as a primary reference standard for investigating the regulation of NAD+ metabolic flux, cellular methylation dynamics, and mitochondrial respiration across diverse cell lines:

• Selective NNMT Enzyme Inhibition: Blocks the methyltransfer from S-adenosylmethionine (SAM) to nicotinamide (NAM), preventing the depletion of intracellular NAM and redirecting it into the NAD+ salvage pathway.
• Intracellular NAD+ Elevation & Sirtuin Activation: Increased NAD+ availability stimulates the catalytic activity of NAD+-dependent deacetylases (particularly SIRT1), inducing downstream activation of the transcriptional coactivator PGC-1$alpha$ and promoting mitochondrial biogenesis in vitro.
• Modulation of Adipocyte Differentiation & Lipid Handling: NNMT inhibition in preadipocytes and mature adipocytes downregulates key lipogenic transcriptional programs, limits intracellular lipid droplet accumulation, and enhances basal cellular metabolic rate in vitro.
• Myogenic Stem Cell Proliferation in Muscle Models: In senescence and muscle injury cell models in vitro, it enhances the proliferative and myogenic fusion capacity of satellite cells, facilitating myotube formation.

Technical & Chemical Specifications

• Classification: Selective NNMT Inhibitor / Small Molecule Standard in Solid Matrix (RUO)
• Chemical Name: 5-amino-1-methylquinolin-1-ium
• Molecular Formula: C10H11N2+
• Molecular Weight: 159.21 g/mol (free cation)
• Active Content: 50 mg per capsule (±2%)
• Packaging Unit: 60 research capsules in a laboratory container with integrated desiccant
• Form: Solid reagent matrix / encapsulated standard
• Component Identification: Verified via Analytical HPLC and Mass Spectrometry (MS / NMR)
• Recommended Reconstitution Protocol: Capsule contents are intended for dissolution in suitable laboratory solvents (e.g., DMSO for concentrated stock solutions or sterile laboratory buffers) to prepare calibrated working solutions for in vitro cellular assays.
• Storage Conditions: Store at 15–25 °C in a dry, dark environment in the tightly sealed original container with desiccant. For long-term archiving of analytical standards, storage at -20 °C is recommended.

Scientific References & Literature

• Neelakantan, D., et al. Small molecule nicotinamide N-methyltransferase inhibitor activates senescent muscle stem cells and enhances myogenesis in vitro. Biochemical Pharmacology, 2019.
• Kraus, D., et al. Nicotinamide N-methyltransferase knockdown protects against diet-induced metabolic dysfunction and regulates cellular energy expenditure. Nature, 2014.
• Harshman, S. W., et al. Characterization of small molecule NNMT inhibitors in regulating NAD+ salvage flux and sirtuin signaling pathways. Cell Chemical Biology, 2018.
• Campagna, R., et al. The role of NNMT in metabolic and epigenetic reprogramming: In vitro characterization of novel quinolinium-based inhibitors. Frontiers in Cell and Developmental Biology, 2021.

Legal Disclaimer & Terms of Purchase

This product is classified and supplied strictly for laboratory, scientific, and in vitro research purposes only (Research Use Only – RUO). Neither the compound nor the capsule matrix is a drug, medicine, dietary supplement, cosmetic product, or substance intended for human or veterinary consumption, oral ingestion, direct administration, injection, or diagnostic procedures.

By placing an order, the purchaser explicitly confirms that they are at least 18 years of age, possess the requisite professional qualifications and laboratory equipment to safely handle research materials, and are fully conversant with all relevant safety protocols for managing research substances. The seller assumes no liability for any use contrary to these terms.