Description

PEG-MGF (Pegylated Mechano-Growth Factor)

Synthetic bioactive polyethylene glycol-conjugated C-terminal peptide isoform of IGF-1 (PEG-IGF-1Ec / E-peptide) for in vitro research on prolonged stability, skeletal muscle satellite cell activation, cardioprotection, and sustained paracrine signaling

Overview & Mechanism of Action (In Vitro)

PEG-MGF (Pegylated Mechano-Growth Factor / Pegylated IGF-1Ec fragment) is a synthetic C-terminal peptide domain of human MGF (typically corresponding to the 24–45 amino acid sequence of the IGF-1Ec E-domain), covalently conjugated at its N-terminus with a polyethylene glycol polymer chain (PEGylation). While native unmodified MGF possesses an in vitro half-life of only several minutes due to rapid enzymatic degradation by proteases, PEGylation creates a protective hydration shell that impedes proteolysis, significantly prolonging biological stability in culture media and enabling sustained receptor interaction:

• Sustained Activation & Expansion of Satellite Cells: Delivers continuous stimulation of quiescent myogenic stem cells (satellite cells) in injured or mechanically stressed muscle cultures, enhancing long-term proliferative capacity in vitro.
• Specialized E-Domain Signaling Pathway: Acts via specific binding sites distinct from the classical systemic IGF-1 receptor (IGF-1R), activating intracellular Akt/mTOR and ERK1/2 kinase pathways to drive protein synthesis without cross-activating the insulin receptor.
• In Vitro Cardioprotection & Neuroprotection: Shields cardiomyocytes and neural cell lines against oxidative stress, hypoxia-induced apoptosis, and nutrient-deprivation injury.
• Extracellular Matrix Remodeling & Senescence Reduction: Modulates tissue-repair transcription factors, attenuates cellular senescence, and supports progenitor cell viability in extended cell culture assays.

Technical & Chemical Specifications

• Classification: Research Peptide / Pegylated C-Terminal IGF-1Ec Fragment (PEG-MGF)
• Core Peptide Sequence: PEG-Tyr-Gln-Pro-Pro-Ser-Thr-Asn-Lys-Asn-Thr-Lys-Ser-Gln-Arg-Arg-Lys-Gly-Ser-Thr-Phe-Glu-Glu-His-Lys-NH2
• Modification: N-terminal covalent conjugation with linear polyethylene glycol (typically PEG 2 kDa or batch-specified)
• Form: Sterile lyophilized white powder (cake)
• Component Identification: Verified via MALDI-TOF Mass Spectrometry and analytical HPLC
• Recommended Reconstitution Solvent: Sterile bacteriostatic water or sterile phosphate-buffered saline (PBS, pH 7.2–7.4)
• Storage Conditions: Store long-term at -20 °C in a dry, dark environment. Following reconstitution, keep refrigerated at 2–8 °C in accordance with standard laboratory protocols.

Scientific References & Literature

• Dluzniewska, J., et al. A strong neuroprotective effect of MGF E-peptide in in vitro and in vivo models of cerebral ischemia. The FASEB Journal, 2005.
• Goldspink, G., et al. Pegylated IGF-I E-peptide exhibits extended bioactivity and promotes muscle stem cell proliferation. Biochemical Society Transactions, 2007.
• Mills, P., et al. Mechanical stimulus and the regulation of MGF splice variants: Effects of pegylation on functional longevity. Journal of Applied Physiology, 2008.
• Carpenter, V., et al. Cardioprotective properties of the IGF-1Ec C-terminal fragment under simulated hypoxic stress. Cardiovascular Research, 2009.

Legal Disclaimer & Terms of Purchase

This product is classified and supplied strictly for laboratory, scientific, and in vitro research purposes only (Research Use Only – RUO). This compound is not a drug, medicine, dietary supplement, cosmetic product, or substance intended for human or veterinary consumption, direct administration, injection, or diagnostic procedures.

By placing an order, the purchaser explicitly confirms that they are at least 18 years of age, possess the requisite professional qualifications and laboratory equipment to safely handle research materials, and are fully conversant with all relevant safety protocols for managing research substances. The seller assumes no liability for any use contrary to these terms.